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Highly sensitive ELISA for UV-induced DNA damage

The High Sensitivity CPD and the High Sensitivity (6-4) photoproducts ELISA from Cosmo Bio

Most of the UV-induced DNA damage are cyclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts (6-4PPs), which are formed between neighboring pyrimidine nucleotides on the same DNA strand. These helix-distorting DNA lesions are only repaired in humans by a nucleotide excision repair system.The main types of UV-induced DNA damage are cyclobutane pyrimidine dimers (CPDs), (6-4) photoproducts (6-4PPs) and dewar photoproducts (DewarPPs), which are formed between neighboring pyrimidine nucleotides on the same DNA strand become.

Cyclobutane pyrimidine dimers (CPDs), (6-4) photoproducts (6-4PPs) and Dewar photoproducts (DewarPPs) are UV-induced DNA damage that are formed between neighboring pyrimidine nucleotides on the same DNA strand.

The High Sensitivity CPD / Cyclobutane Pyrimidine Dimer ELISA kit ( CSR-NM-MA-K003 ) from Cosmo Bio is the only commercially available ELISA using the anti-CPD clone TDM-2 and was developed for the highly sensitive detection of CPDs in cultured cells or DNA purified from the skin epidermis.

The High Sensitivity (6-4) photoproducts (6-4PPs) ELISA Kit ( CSR-NM-MA-K002 ) is the only commercially available ELISA that uses the anti-6-4PPs clone 64M-2 and for the highly sensitive one Detection of 6-4PPs from cultured cells from the skin epidermis was optimized.

This ELISA recognizes CPDs or 6-4PPs from dipyrimidines in all DNA sequence contexts (eg TT, TC, CT and CC). Therefore, the availability and convenience of this ELISA kit will help to understand the molecular mechanisms of cellular responses to UV radiation and DNA damage with applications in many areas of research such as cancer research, photobiology, dermatology, ophthalmology, immunology and cosmetic science.

To better investigate molecular events in the environment of UV-induced DNA damage and repair, Mori et al. previously developed and characterized monoclonal antibodies (mAb) specifically for CPDs and mAb specifically for 6-4PPs (1), while Matsunaga et al. mAb developed and characterized specifically for Dewar photo products (DewarPPs) (2).

Three of these antibodies (CPDs: clone TDM-2 [ CAC-NM-DND-001 ]; 6-4PPs: clone 64M-2 [ CAC-NM-DND-002 ]; DewarPPs: clone DEM-1 [ CAC-NM-DND -003 ]) continue to be widely cited in the literature, often for use in ELISA by a recommended method. These antibodies enable the quantification of photoproducts in purified DNA, those from cultured cells or from the skin epidermis using an enzyme-linked immunoadsorption test (ELISA) and the visualization and measurement of photoproducts in the DNA from cultivated cells or the skin by means of indirect immunofluorescence (IIF).

credentials

1) Mori, T., et al., Photochem. Photobiol. 54, 225-232 (1991).

2) Matsunaga, T., et al., Photochem. Photobiol. 54, 403-410 (1991).

Blogging

Cell lines in the life sciences

Cell lines are an integral a part of the life sciences

Cell lines are discovering growing purposes in biology and the life sciences. They have a everlasting place in analysis and improvement. They are important in the manufacture of molecular merchandise akin to monoclonal antibodies, vaccines, enzymes and lots of others. In addition to those established purposes, they’re additionally used in new areas. One of those areas is “Cellular Agriculture”, which offers with the manufacturing of agricultural merchandise utilizing cell cultures.

Establishment of a cell line

The cultivation of cells takes place in a nutrient medium outdoors the organism. A distinction is made between immortalized lines and first cultures. The main tradition describes a non-immortalized cell tradition, which was obtained instantly from the tissue. In order to efficiently convey cells into tradition, the alternative of the tradition medium is of explicit significance. Among different issues, the pH worth and the buffer system have to be chosen appropriately. The cells should even be provided with the correct quantity of amino acids, nutritional vitamins and salts. Furthermore, the temperature and the oxygen and carbon dioxide focus have to be set optimally.

Frequently used cell lines

A complete of over 5000 cell lines are listed in the American Type Culture Collection (ATCC) and the European Collection of Cell Cultures (ECACC). There you possibly can request vital info and areas of utility to plan your individual analysis. At the second, research are being carried out on varied sorts of most cancers, but additionally in primary analysis (e.g. embryonic improvement and plant breeding). Some cell lines are used significantly ceaselessly for this goal.

  • HEK cells , abbreviated for “Human Embryonic Kidney” cells, are sometimes used because of their easy cultivation. They are reworked cells which include overseas DNA from human adenovirus 5. The hypotriploid epithelial cells grow to be adherent and are sometimes used in the improvement of chemotherapy medicine.
  • HeLa cells (named after the affected person Henrietta Lacks) are most likely the most ceaselessly used cell line. In 1951 the first cells have been taken from the line. Since then, working with HeLA cells has revealed over 75,000 scientific articles and 4 Nobel Prizes. The analysis offered findings in most cancers analysis, gene mapping and the functioning of polymerase enzymes.
  • HaCaT cells  are a spontaneously reworked aneuploid, immortal keratinocyte cell line, which originates from grownup human pores and skin. HaCaT cells are recognized for his or her means to distinguish in vitro. The outcomes obtained are extra reproducible.