Goat anti-Mouse Gold (40nm)
Antibody Format: Whole IgG
Specificity: IgG (H+L)
Minimal cross-reactivity: Human, bovine, horse, rabbit and rat serum proteins
Conjugate: 40nm colloidal gold
Product Category: 40nm ImmunoGold for LFIA
OD at 529nm: 10.0 (see spec sheet)
Estimated Delivery: 5-7 days
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. Antibodies against non-immunoglobulin serum proteins were not detected. The antibody has been tested by ELISA and/or solid-phase adsorption to ensure minimal cross-reaction with human, bovine, horse, rabbit and rat serum proteins, but may cross-react with immunoglobulins from other species.
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have one Fc portion and two antigen-binding Fab portions linked by disulfide bonds and are therefore divalent. The average molecular weight is reported to be about 160 kDa. The complete form of IgG antibodies is suitable for most immunodetection procedures and is the most cost-effective.
Physical State: Sterile filtered liquid
Storage: Store at 2-8°C. Prepare the working dilution on the day of use.
Expiration date: one year from the date of receipt. The expiration date may be extended if the test results are acceptable for the intended use.
Purity: Antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 2.0 mM sodium borate, pH 9.0
Preservative: 0.05% sodium azide
Suggested Working Concentration or Dilution Range: 1:10 – 1:100
Colloidal gold conjugates are widely used commercially in lateral flow immunoassays due to their ease of production, conjugate stability, and strong optical properties that generate highly sensitive tests with results visible to the naked eye. Conjugates are prepared by passive absorption of the antibody against the gold particle and sterile filtered into a low ionic strength buffer containing a preservative.