A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers., azide free, Blogging, elisa, Goat, Hamster, Mammal, Mink, Mouse, Multiplexed shotgun genotyping for rapid and efficient genetic mapping., NGS QC Toolkit: a toolkit for quality control of next generation sequencing data., Opossum, Orangutan, pcr, Pig, Pigeon, Plant, Rat, Yeast

Molecular Profiling of Malignant Pleural Effusions with Next Generation Sequencing (NGS): Evidence that Supports Its Role in Cancer Management

Malignant pleural effusions (MPEs) typically develop in superior most cancers sufferers and confer important morbidity and mortality. In this overview, we evaluated whether or not molecular profiling of MPEs with subsequent technology sequencing (NGS) may have a task in most cancers administration, specializing in lung most cancers. We reviewed and in contrast the diagnostic efficiency of pleural fluid liquid biopsy with different sorts of samples. When utilized in MPEs, NGS could have comparable efficiency with corresponding tissue biopsies, yield increased DNA quantity, and detect extra genetic aberrations than blood-derived liquid biopsies.

Of curiosity, post-centrifuge supernatant NGS could exhibit superior outcomes in comparison with cell pellet, cell block or different supplies. NGS in MPEs can even information clinicians in tailoring established therapies and figuring out remedy resistance. Evidence remains to be untimely concerning the position of NGS in MPEs from sufferers with cancers aside from lung. We concluded that MPE processing may present helpful prognostic and theranostic data, moreover its diagnostic position. Detecting persistent minimal residual illness (MRD) permits the identification of sufferers with an elevated danger of relapse and demise.

In this research, we have now evaluated MRD three months after transplantation in 106 myeloma sufferers utilizing a industrial next-generation sequencing (NGS) technique (LymphoTrack®), and in contrast the outcomes with next-generation circulate (NGF, EuroFlow). The use of totally different marrow pulls and the necessity of concentrating samples for NGS biased the applicability for MRD analysis and favored NGF. Hypophosphataemic rickets (HR) comprise a clinically and genetically heterogeneous group of situations, outlined by renal-tubular phosphate losing and consecutive loss of bone mineralisation.

X-linked hypophosphataemia (XLH) is the most typical type, attributable to inactivating dominant mutations in PHEX, a gene encompassing 22 exons situated at Xp22.1. XLH is treatable by anti-Fibroblast Growth Factor 23 antibody, whereas for different kinds of HR akin to remedy will not be indicated. Therefore, a genetic differentiation of HR is beneficial. NGS in MPEs may be preferable to plasma liquid biopsy in superior most cancers sufferers with a MPE and a paucicellular or it could possibly be tough to acquire tissue/fine-needle aspiration biopsy.

Assessing Nanopore sequencing for medical diagnostics: A comparability of NGS strategies for Mycobacterium tuberculosis

Next-generation sequencing applied sciences are being quickly adopted as a software of alternative for diagnostic and outbreak investigation in public well being laboratories. However, prices of operation and the necessity for specialised employees stay main hurdles for laboratories with restricted sources for implementing these applied sciences. This mission aimed to evaluate the feasibility of utilizing Oxford Nanopore MinION whole-genome sequencing information of Mycobacterium tuberculosis isolates for species identification, in silico spoligotyping, detection of mutations related with antimicrobial resistance (AMR) to precisely predict drug susceptibility profiles, and phylogenetic evaluation to detect transmission between circumstances.

The outcomes have been in contrast prospectively in real-time, to these obtained with our present clinically validated Illumina MiSeq sequencing assay for M. tuberculosis and phenotypic drug susceptibility testing outcomes when obtainable. Our evaluation of 431 sequenced samples over a 32-week interval demonstrates that, when utilizing the correct qc and thresholds, the MinION can obtain ranges of genotyping evaluation and phenotypic resistance predictions corresponding to these of the Illumina MiSeq, at a really aggressive price per pattern.

Our outcomes point out that nanopore sequencing generally is a appropriate various to, or complement, at the moment used sequencing platforms in a medical setting and has the potential to be broadly adopted in public well being laboratories in the close to future. We designed an AmpliSeq gene panel for the IonTorrent PGM next-generation platform for PHEX and ten different HR-related genes. For validation of PHEX sequencing 50 DNA-samples from XLH-patients, in whom 42 totally different mutations in PHEX and 1 structural variation have been confirmed earlier than, have been blinded, anonymised and investigated with the NGS panel.

Molecular Profiling of Malignant Pleural Effusions with Next Generation Sequencing (NGS): Evidence that Supports Its Role in Cancer Management

Advancing leukemia diagnostics: Role of Next Generation Sequencing (NGS) in acute myeloid leukemia

AML diagnostics, initially primarily based solely on morphological analysis, now depends on a number of disciplines to succeed in its full potential. Only by integrating the outcomes of cytomorphology, cytochemistry, immunophenotyping, cytogenetics and molecular genetics it’s doable to fulfil WHO classification and ELN prognostication programs. Especially molecular genetics has gained rather a lot of curiosity during the last decade, primarily by the introduction of subsequent technology sequencing (NGS). NGS utility ranges from the investigation of single genes and panels to even complete exomes, transcriptomes and genomes. In routine AML diagnostics panels are the popular NGS methodology.

Here, we are going to overview the ability and limitations of NGS in the context of prognosis, prognosis and precision drugs. Due to excessive dimensionality, NGS information interpretation is difficult but it surely additionally presents a novel investigatory probability and the chance to use information mining methods akin to synthetic intelligence. We will even mirror on how the incorporation of the improved data base into routine diagnostics can pave the best way for higher remedy and extra remedy in AML. Hereditary hemolytic anemias current a novel diagnostic problem on account of their broad phenotypic and genotypic spectrum. Accurate prognosis is important to make sure acceptable remedy.

WUV-M20, UV Transilluminator, 312nm, 220V

3532198 1unit
EUR 1627

uv transilluminator 20x20cm, 312nm, 50/100% switch

UVTR2200 ea
EUR 1161

BLook: LED Transilluminator

BK001 1 pcs Ask for price

transilluminator led 20x12 cm + hood

BLOOK ea
EUR 580

ENDURO™ PowerStack

LE0304S Ea
EUR 604

Fluorescent UV Particles

FP-15040-2 2 mL
EUR 283
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

UV TRANSMITTING TRAY

GD-GCS 1/pk
EUR 208
Description: Lab Equipment; Axygen Branded EQ

uv protected glasses

VLLP70 ea
EUR 99

SmartDoc UV Blocking Mat

E5000-MAT 1 PC
EUR 100.67
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

uv lamp 1x15w, 254nm

VL115-C ea
EUR 656

uv lamp 1x15w, 365nm

VL115-L ea
EUR 656

uv lamp 1x15w, 312nm

VL115-M ea
EUR 656

uv lamp 2x15w, 254nm

VL215-C ea
EUR 792

uv lamp 2x15w, 365nm

VL215-L ea
EUR 792

uv lamp 2x15w, 312nm

VL215-M ea
EUR 817

uv lamp 1x6w, 254nm

VL6-C ea
EUR 521

uv lamp 1x6w, 365nm

VL6-L ea
EUR 521

conversion screen uv/blue

VLFC26-BLUE ea
EUR 606

conversion screen uv/wl

VLFC26-WL ea
EUR 606

uv protected face shield

VLMP80 ea
EUR 183

uv protected face shield

VLMP800 ea
EUR 200

ENDURO™ 250V Power Supply , 120V

LE0203-120 Ea
EUR 1603

ENDURO™ 250V Power Supply , 230V

LE0203-230 Ea
EUR 1603

ENDURO™ 300V Power Supply , 120V

LE0303-120 Ea
EUR 1198

nUVaClean UV pipette carousel, with UV lamp, for 6 universal pipettes,

P5590 1/pack
EUR 356.25
Description: nUVaClean UV pipette carousel with UV lamp

nUVaClean UV pipette carousel, with UV lamp, for 6 universal pipettes,

P5590-E 1/pack
EUR 356.25
Description: nUVaClean UV pipette carousel with UV lamp

UV Carboxyl Particle Array Kit

UVCPAK--5042-14K 14X1 mL
EUR 1278
Description: UltraRainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

uv lamp 2x15w, 254/365nm

VL215-LC ea
EUR 817

uv lamp 2x15w, 312/365nm

VL215-LM ea
EUR 817

uv lamp 2x15w, 254/312nm

VL215-MC ea
EUR 859

uv lamp 2x6w, 254/365nm

VL6-LC ea
EUR 538

starter uv tube 4w-20w

VLST151 ea
EUR 56

Casting Dams, For Enduro 7cm Gel System

LE1000CD-01 Ea
EUR 126

Casting Dams, For Enduro 10cm Gel System

LE1000CD-02 Ea
EUR 138

Casting Dams, For Enduro 15cm Gel System

LE1000CD-03 Ea
EUR 165

Casting Dams, For Enduro 20cm Gel System

LE1000CD-04 Ea
EUR 192

uv table 8w 254nm 15x15 cm

VLECX-15C ea
EUR 1028

uv table 8w 312nm 15x15 cm

VLECX-15M ea
EUR 1028

uv table blue led 20x20 cm

VLECX-20BLUE ea
EUR 1535

uv table 8w 254nm 20x20 cm

VLECX-20C ea
EUR 1113

uv table 8w 365nm 20x20 cm

VLECX-20L ea
EUR 1113

uv table 8w 312nm 20x20 cm

VLECX-20M ea
EUR 1163

uv table 8w, 254nm 21x26 cm

VLECX-26C ea
EUR 1197

uv table 8w, 312nm 21x26 cm

VLECX-26M ea
EUR 1248

uv tube 15 w, 254 nm

VLT15-C ea
EUR 56

uv tube 15 w, 365 nm

VLT15-L ea
EUR 56

uv tube 15 w, 312 nm

VLT15-M ea
EUR 82

uv tube 4 w, 254 nm

VLT4-C ea
EUR 56

uv tube 4 w, 365 nm

VLT4-L ea
EUR 56

uv tube 6 w, 254 nm

VLT6-C ea
EUR 56

uv tube 6 w, 365 nm

VLT6-L ea
EUR 56

uv tube 8 w, 254 nm

VLT8-C ea
EUR 56

uv tube 8 w, 365 nm

VLT8-L ea
EUR 56

uv tube 8 w, 312 nm

VLT8-M ea
EUR 82

Absolute Mag Magnetic Particles, Fluorescent UV

WHM-S135 2 mL
EUR 604

ENDURO™ Mini 300V Power Supply , 110V-220V

LE0304 Ea
EUR 705

ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels

LE1001 Set
EUR 543

ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels

LE1002 Set
EUR 583

ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels

LE1003 Set
EUR 489

ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels

LE1004 Set
EUR 739

ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels

LE1005 Set
EUR 901

ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels

LE1006 Set
EUR 975

Uv Radiation Resistance Associated Gene (UVRAG) Antibody

20-abx116558
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

PLT,96WL,UV,NT,NS,25/50

3635 25/pk
EUR 545
Description: Genomics; Genomic Plates

WSE-5300 Printgraph CMOS I 6M UV

2305300 1unit
EUR 8510
Description: I ncl udes 312 nm U V t r ansi l l umi nat or , Whi t e L EDl i ght pl at e CE marked

Bacillus cereus UV DNA damage endonuclease (uvsE)

1-CSB-EP467668BQP
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 44.0 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Bacillus cereus UV DNA damage endonuclease(uvsE) expressed in E.coli

uv table 8w 365/254nm 20x20 cm

VLTCP-20LC ea
EUR 1282

uv table 8w 365/312nm 20x20 cm

VLTCP-20LM ea
EUR 1282

uv table 8w 312/254nm 20x20 cm

VLTCP-20MC ea
EUR 1282

uv table 2x8w, 365/254nm 21x26 cm

VLTCP-26LC ea
EUR 1620

uv table 2x8w, 365/312nm 21x26 cm

VLTCP-26LM ea
EUR 1620

uv table 2x8w, 312/254nm 21x26 cm

VLTCP-26MC ea
EUR 1620

Standard UV Quartz Cell with Teflon Stopper

SQ014 1 UNIT
EUR 147.24
  • Product category: Labware/Cuvettes/Quartz Cells

12 Well Comb ,0.75mm Thick For Enduro 10cm Gel System

LE1000C10-03 Ea
EUR 128

12 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-04 Ea
EUR 128

12 Well Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-05 Ea
EUR 128

16 Well Comb ,0.75mm Thick For Enduro 10cm Gel System

LE1000C10-06 Ea
EUR 128

16 Well Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-07 Ea
EUR 128

16 Well Comb ,2mm Thick For Enduro 10cm Gel System

LE1000C10-08 Ea
EUR 128

25 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-11 Ea
EUR 128

8 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-12 Ea
EUR 128

10 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-01 Ea
EUR 131

10 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-02 Ea
EUR 131

15 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-03 Ea
EUR 131

14 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-04 Ea
EUR 131

16 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-05 Ea
EUR 131

20 Well Comb ,0.75mm Thick For Enduro 15cm Gel System

LE1000C15-10 Ea
EUR 131

20 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-11 Ea
EUR 131

20 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-12 Ea
EUR 131

20 Well Comb ,2mm Thick For Enduro 15cm Gel System

LE1000C15-13 Ea
EUR 131

8 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-17 Ea
EUR 131

16 Well Comb ,0.75mm Thick For Enduro 20cm Gel System

LE1000C20-01 Ea
EUR 138

25 Well Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-05 Ea
EUR 138

30 Well Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-06 Ea
EUR 138

50 Well Comb ,2mm Thick For Enduro 20cm Gel System

LE1000C20-10 Ea
EUR 138

10 Well Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-01 Ea
EUR 124

10 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-02 Ea
EUR 124

16 Well Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-05 Ea
EUR 124

16 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-06 Ea
EUR 124

5 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-07 Ea
EUR 124

8 Well Comb , 0.75mm Thick For Enduro 7cm Gel System

LE1000C7-08 Ea
EUR 124

8 Well Comb ,1mm Thick For Enduro 7cm Gel System

LE1000C7-09 Ea
EUR 124

UV excision repair protein RAD23 homolog A Antibody

20-abx110497
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody

20-abx006162
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody

abx030355-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody

abx030355-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody

abx030356-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody

abx030356-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody

abx030357-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody

abx030357-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody

abx030358-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody

abx030358-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody

20-abx313923
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody

abx448488-100ug 100 ug
EUR 523
  • Shipped within 5-12 working days.

UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody

abx448489-100ug 100 ug
EUR 523
  • Shipped within 5-12 working days.

UV Radiation Resistance-Associated Gene Protein (UVRAG) Antibody

abx239352-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

One-Step Lumitein UV Protein Gel Stain, 1X

21005-1L 1L
EUR 222
Description: Minimum order quantity: 1 unit of 1L

One-Step Lumitein UV Protein Gel Stain, 1X

21005-4L 4L
EUR 567
Description: Minimum order quantity: 1 unit of 4L

One-Step Lumitein UV Protein Gel Stain, 1X

21005-F 25mL
EUR 50
Description: Minimum order quantity: 1 unit of 25mL

uv table 8w 254nm/wl 2x(20x20) cm

VLTFP-CWL ea
EUR 1535

uv table 8w 365nm/wl 2x(20x20) cm

VLTFP-LWL ea
EUR 1535

uv table 8w 312nm/wl 2x(20x20) cm

VLTFP-MWL ea
EUR 1535

Replacement power supply for nUVaClean UV Pipette Carousel

P5590-PS 1/pack
EUR 76.33
Description: Replacement power supply for nUVaClean UV Pipette Carousel

BioWand Personal UV Sanitizer, 265-280nm, 1/ea

U1001-W 1/pack
EUR 96.58
Description: BioWand Personal UV Sanitizer 265-280nm

10 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-01 Ea
EUR 128

10 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-02 Ea
EUR 128

20 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-09 Ea
EUR 128

20 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-10 Ea
EUR 128

16 Well Mc Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-06 Ea
EUR 131

16 Well Mc Comb ,2mm Thick For Enduro 15cm Gel System

LE1000C15-07 Ea
EUR 131

18 Well Mc Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-08 Ea
EUR 131

18 Well Mc Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-09 Ea
EUR 131

28 Well Mc Comb ,0.75mm Thick For Enduro 15cm Gel System

LE1000C15-14 Ea
EUR 131

28 Well Mc Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-15 Ea
EUR 131

30 Well Mc Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-16 Ea
EUR 131

20 Well Mc Comb ,0.75mm Thick For Enduro 20cm Gel System

LE1000C20-02 Ea
EUR 138

20 Well Mc Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-03 Ea
EUR 138

20 Well Mc Comb ,1.5mm Thick For Enduro 20cm Gel System

LE1000C20-04 Ea
EUR 138

40 Well Mc Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-07 Ea
EUR 138

40 Well Mc Comb ,1.5mm Thick For Enduro 20cm Gel System

LE1000C20-08 Ea
EUR 138

40 Well Mc Comb ,2mm Thick For Enduro 20cm Gel System

LE1000C20-09 Ea
EUR 138

12 Well Mc Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-03 Ea
EUR 124

We report two circumstances, which offered as hemolytic anemias, however preliminary workup was inconclusive they usually have been lastly recognized with the assistance of Next Generation Sequencing (Dehydrated Hereditary Stomatocytosis and Kӧln Hemoglobinopathy). In addition, we analyzed one recognized homozygous DMP1 mutation and two samples of HR-patients, the place no pathogenic PHEX mutation had been detected by typical sequencing.